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Agrisera/DYKDDDDK (binds to Sigma FLAG®) (mouse monoclonal)/AS15 3036/
product information
Background

Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).

Immunogen

KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®)

Host Mouse
Clonality Monoclonal
Clone Clone 1E6
Purity protein G purified
Format Liquid in PBS, pH 7.4, with 0.05% sodium azide
Quantity 100 µg
Reconstitution
Storage

Antibody can be stored at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Related products

AS15 2871 | anti-DYKDDDDK (binds to Sigma FLAG®), mouse monoclonal antibodyAS15 2952 | anti-DYKDDDDK (binds to Sigma FLAG®), ALP conjugated, mouse monoclonal antibody

AS15 2950 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 488 conjugated (mouse antibody)

AS15 2951 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 550 conjugated (mouse antibody)

AS15 2947 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 650 conjugated (mouse antibody)AS15 2948 | anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated (mouse antibody)

AS15 2949 | anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (mouse antibody)AS15 3037 | anti-DYKDDDDK (binds to Sigma FLAG®), (rabbit antibody)

other tag antibodies

Secondary antibodies

Additional information

exact working dilution needs to be determined by end user

application information
Recommended dilution

1:100 (WB), 1:20 (IP), 1:20 (IHC)

Expected | apparent MW
Confirmed reactivity DYKDDDDK epitope tag, fused to proteins in mammalian cells
Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information protein G purified
Selected references Wardhan et al. (2017). Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure. Sci Rep. 2016 Dec 9;6:38121. doi: 10.1038/srep38121.