DESCRIPTION:
A fully validated, highly specific rabbit polyclonal anti-serum to Serine-197 phosphorylated forms Calcineurin
Cytoplasmic Ca2+ binds to calmodulin and activates the phosphatase calcineurin (Cn) a 60 kDa protein, and CaM kinase II (CaMKII). One action of Cn is to dephosphorylate cytoplasmic NFAT (nuclear factor of activated T cells), inducing its translocation to the nucleus where it activates antiapoptotic and hypertrophic target genes. The phosphorylation of Cn at Phospho Ser-197 site has been observed in cultured neonatal rat ventricular myocytes (NRVMs) over-expressing constitutively active CAMKII. This appears to inhibit Cn activity which in turn prevents the dephosphorylation of NFAT and prevents activation of NFAT regulated genes.
Tested applications: Western blot (1:200 dilution). Species recognition: The antibody recognises Calcineurin in mouse/rat/human/monkey/other, when phosphorylated on Ser-197.
DATA SHEET:
Description | Lyophilised rabbit polyclonal anti-serum containing IgG antibody specific for Ser-197 phosphorylated forms Calcineurin |
Immunogen | Synthetic peptide CGKMARVFS(PO3H2)VLREES-amide corresponding to residues surrounding Ser at position 197 of Calcineurin |
Isotype | IgG |
Purification | Raw serum |
Species reactivity | The antibody recognises Calcineurin in all mammalian species, when phosphorylated on Ser-197. Eptiope sequence also aligns with chicken and Xenopus |
Tested applications | WB |
Recommended dilution | WB: 1:200 |
Optimization | Optimal dilutions to be determined by end user |
Storage | Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles. |
Storage | Lyophilised: 4 °C. Reconstituted:-80 °C |
Datasheet | A010-80 |
MSDS | A010-80 |
Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |