DESCRIPTION:

A fully validated, highly specific affinity purified rabbit antibody to threonine-17 phosphorylated forms of phospholamban

Phospholamban (PLB/PLN) is a small transmembrane protein which plays an important role in controlling the activity of the sarcoplasmic reticulum ATPase (SERCA2a) of cardiac muscle during calcium sequestration.

Phospholamban is phosphorylated on separate amino acid residues by cAMP-dependent, and cGMP-dependent (Ser-16)and Ca²⁺/CaM-dependent (threonine-17) protein kinases in response to β-adrenergic stimulation. Akt has also been shown to phosphorylate threonine-17. The result is an increased calcium pump activity which reduces the time course of the calcium transient, increases the calcium load in the sarcoplasmic reticulum, and consequently, produces a larger calcium transient at the next action potential. However, alteration in this homeostatic interaction has been shown to result in heart failure.

Tested applications:Western blot (1:5000 dilution), IHC microscopy (1:200 dilution).

DATA SHEET:

Description	Lyophilised Rabbit polyclonal anti-serum (A010-13) containing IgG antibody specific for Thr-17 phosphorylated forms of PLB (Drago & Colyer, 1994).
Immunogen	Phosphopeptide comprising residues 9-19-Y (residues R9SAIRRAST(PO3H2)IEY20) conjugated to KLH.
Isotype	IgG
Purification	Raw serum
Species reactivity	Reacts with Phospho Thr-17 of phospholamban from Human, mouse, rat, rabbit, chicken, bovine, canine, ferret, hamster and sheep
Tested applications	WB, IHC, ELISA
Recommended dilution	WB 1:5000, IHC 1:200,
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 20 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4 °CReconstituted:-80 °C
Datasheet	A010-13
MSDS	A010-13
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.