DESCRIPTION:

A fully validated, highly specific affinity purified mouse monoclonal antibody to SERCA isoforms 1 and 2 (anti-SERCA1/2)

SERCA1 is the Ca2+-pump (109kDa) located in the sarcoplasmic reticulum (SR) of fast-twitch skeletal muscle and SERCA2 is the Ca2+-pump located in the sarcoplasmic reticulum (SR) of cardiac, smooth and slow-twitch skeletal muscle. The enzyme transports 2 Ca2+ from the cytoplasm into the SR using the hydrolysis of 1 molecule of ATP to power the reaction. This terminates muscle contraction and promotes muscle relaxation. Mutations in SERCA1 are associated with Brody disease; mutations in SERCA2 are associated with Darier disease and down regulation of SERCA2 is seen in heart failure. The monoclonal antibody Y/1F4 was produced by immunizing mice with purified SERCA1: the antibody inhibits SERCA1 activity upon binding (Colyer et al., 1989). Antibody Y/1F4 recognises both SERCA1 and SERCA2.

Tested Applications: Western blot (1:2000 dilution), IHC Microscopy (1:50 dilution). Species recognition: Tested on rat tissue, but immunogenic peptide aligns with Mammalian, Avian, Amphibian and Fish SERCA

DATA SHEET:

Description	Affinity purified mouse monoclonal antibody to SERCA isoforms 1 and 2
Immunogen	Synthetic peptide (VGNKMFVKGA) corresponding to amino acids 508 - 517 of SERCA1, conjugated to keyhole limpet hemocyanin (KLH) by carbodiimide cross linkage.
Isotype	IgG
Purification	Protein A affinity purified
Species reactivity	Tested on Rat tissue, but immunogenic peptide aligns with Mammal, Avian, Amphibian and Fish SERCA
Tested applications	WB
Recommended dilution	WB 1:2000
Optimization	Optimal dilutions to be determined by end user
Storage	Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.
Storage	Lyophilised: 4 °C. Reconstituted:-80 °C
Datasheet	A010-21AP
MSDS	A010-21AP
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.