Biosensis/Mouse monoclonal antibody to DJ-1 [PARK7]: IgG/M-1573-100/100 µg-蚂蚁淘生物

Description	Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinsons disease.
Batch No.	See product label
Unit size	100 µg
Antigen	Full length recombinant human DJ-1 expressed in and purified from E. coli.
Antibody Type	monoclonal
Isotype	IgG1/kappa
Clone	4H4
Other Names	Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1
Accession	Q99497 PARK7_HUMAN;
Produced in	Mouse
Applications	WB, IHC/IF. Suggested dilution of at least 1:1,000 for IHC/IF. Dilutions of 1:10,000 or lower is recommended for WB. This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry.
Species Against	Human, Mouse, Bovine
Antibody Against	DJ1 [PARK7]
Blast URL	Click here
Form	Lyophilized from PBS. Contains 5% trehalose.
Appearance	White powder
Reconstitution	Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage	After reconstitution of lyophilised antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase
General References	1. Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem. Biophys. Res. Commun. 231, 509513 (1997).2. Bonifati V, Rizzu P, van Baren, M.J., Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science, 299, 256259 (2003).3. Xu J, Zhong N, Wang H, Elias JE, Kim CY, Woldman I, Pifl C, Gygi SP, Geula C, Yankner BA. The Parkinsons disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 14 (9):1231-41 (2005).4. Yokota T, Sugawara K, Ito K, Takahashi R, Ariga H. And Mizusawa, H. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. Biochem. Biophys. Res. Commun., 312, 13421348 (2003).5. Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K and Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep., 5, 213218 (2004).6. Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinsons disease. J.Mol,Med.82,163-174 (2004).

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