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Biosensis/Mouse C-C motif chemokine 2 ELISA Kit (2 plates)/BEK-2186-2P/1 Ea
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Description The mouse C-C motif chemokine 2 (Ccl2) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Ccl2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Ccl2 concentration are added to these wells and allowed to complex with the bound Ccl2 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Ccl2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Ccl2 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No. See product labels
Other Names C-C motif chemokine 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Platelet-derived growth factor-inducible protein JE;Small-inducible cytokine A2; Ccl2; Je; Mcp1; Scya2;
Accession P10148 CCL2_MOUSE;
Specificity C-C motif chemokine 2
Storage Store at 4°C
Kit components The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions.
Range 15.6 pg/ml - 1,000 pg/ml
Sensitivity < 6 pg/ml
Kit protocol Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit.