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Biosensis/Goat antibody to beta NGF: whole serum/G-185-100/100 µl
Description FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Batch No. See product label
Unit size 100 µl
Antigen Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Other Names Beta-nerve growth factor
Accession NGF_MOUSE
Produced in Goat
Purity Whole serum
Applications IHC, WB and direct ELISA. A dilution range of 1:1000 - 1:5000 is recommended for IHC and WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown.
Cross-reactivity This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. Other species have not yet been tested.
Blast it If you would like to see the shared identity between different species or other proteins follow the link in Accession field, select then the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it.
Form Lyophilised
Reconstitution Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Ebendal, T. et al (1989) J Neurosci Res 22, 223-240.2. Zhou, X. F. et al (1994) J Neurosci Methods 54, 95-102.3. Angeletti, P. U. et al (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75. 4. Hesse K. et al. (1997) Neurosci Lett. Aug 8;231(2):83-6. 5. Miao J et al. (2012) Neurosci Res. Dec;74(3-4):269-76.