Biosensis/NGF Rapid ELISA Kit: Mouse (1 Plate)/BEK-2213-1P/1 Ea-蚂蚁淘生物

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Description	The Biosensis NGF Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards.This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples.Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was < 0.1% when assayed at 25 ng/mL in assay buffer, and not detectable when assayed across the mature NGF calibration range.This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No.	Refer to the product label.
Antigen	FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Other Names	Beta-nerve growth factor; Ngfb
Accession	NGF_MOUSE
Specificity	Mouse
Cross-reactivity	The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assays blank OD readings.
Storage	Store at 4°C
Expiry Date	12 months from purchase.
Kit components	The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, wash and sample buffers and detailed protocols.
Range	3.9 - 250 pg/mL
Sensitivity	Typical limit of detection (LOD) for mouse NGF is 8 pg/mL determined as 150% of the blank value.
Kit protocol	$\"Click$ BEK-2213_as_at_Dec2016.pdf
MSDS	$\"Click$ MSDS_Rapid_ELISA_as_at_Aug2017.pdf

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