Biosensis/Mouse Matrix metalloproteinase-9 ELISA Kit (1 plate)/BEK-2074-1P/1 Ea-蚂蚁淘生物

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Description	The mouse Matrix metalloproteinase-9 (MMP-9) Kit is a sandwich ELISA. The capture antibody is a mouse MMP-9 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known MMP-9 concentration are added to these wells and allowed to complex with the bound MMP-9 antibody. A biotinylated mouse MMP-9 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MMP-9 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No.	See product labels
Other Names	MMP-9; MMP9; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; CLG4B;
Accession	P41245 MMP9_MOUSE;
Specificity	Mouse MMP-9
Storage	Store at 4øC
Specific References	Rohl J (2017) Intracellular trafficking and secretion of matrix metalloproteinases during macrophage migration. PhD Thesis Queensland University of Technology, Australia. Wang Y et al. (2014) Influence of apolipoprotein E, age and aortic site on calcium phosphate induced abdominal aortic aneurysm in mice. Atherosclerosis. 2014 Jul;235(1):204-12. Moxon JV et al. (2014) Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm. Proteomics Clin Appl. 2014 Oct;8(9-10):762-72.
Kit components	The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, substrate buffer and precise instructions.
Range	156 pg/ml - 10,000 pg/ml
Sensitivity	< 20 pg/ml
Kit protocol	$\"Click$ BEK-2074-1P_as_at_Nov2016_protocol.pdf

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