Biosensis/Mouse MIP-2 ELISA Kit (1 plate)/BEK-2179-1P/1 Ea
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Description | The mouse MIP-2 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse MIP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MIP-2 concentration are added to these wells and allowed to complex with the bound MIP-2 antibody. A biotinylated anti-mouse MIP-2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MIP-2 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
Batch No. | See product labels |
Other Names | MP26; Major intrinsic protein; MIP; |
Specificity | MIP-2 |
Storage | Store at 4øC |
Specific References | Kanaya K et al. (2014) Innate immune responses and neuroepithelial degeneration and regeneration in the mouse olfactory mucosa induced by intranasal administration of Poly(I:C). Cell Tissue Res. 2014 Jul;357(1):279-99. |
Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
Range | 15.6 pg/ml -1000 pg/ml |
Sensitivity | < 5 pg/ml |
Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |