Biosensis/Sheep polyclonal antibody to dephosphorylated Catenin beta (28-46): Affinity purified/S-12-蚂蚁淘生物

Description	Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Batch No.	See product label
Unit size	100 µg
Antigen	A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Other Names	Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin;
Accession	P35222 CTNB1_HUMAN;
Produced in	Sheep
Purity	Affinity purified
Applications	Western Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/ml is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/ml is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	The specificity of this antibody has been confirmed by WB against the antigen.
Cross-reactivity	Human; mouse; rat;
Blast it	If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form	Lyophilised from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Reconstitution	Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage	At least 12 months after purchase at 2 - 4°C (lyophilized formulations).After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date	12 months after purchase

友情链接