Cell Synonyms HAOSMC, HBcASMC, HCtASMC, HCASMC, HITASMC, HPASMC, UASMC, HScASMC, Aortic, Brachiocephalic, Carotid, Coronary, Internal Thoracic, Pulmonary, Subclavian, artery smooth muscle Arterial smooth muscle cells are capable of synthesizing collagen, elastin, myosin and glycosaminoglycan. Increased production of connective tissue components, hyperplasia and hypertrophy of intimal smooth muscle cells are found to gradually occlude the vessel lumen in atherosclerosis.Arterysmooth muscle cellsrespond to various factors by proliferating or differentiating. They are a well established cell system for the study of human vascular disorders such as atherosclerosis and stroke. Applications synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines Suitable for Reverse TransfectionCompatible with transfection in any plate formats Economical: High efficiency means less amount of nucleic acid reagent is neededDeveloped and manufactured by EZ Biosystems The transfection reagent work best for the following artery smooth muscle cells: FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human aortic smooth muscle cell (HAOSMC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HAOSMC. This test result was also confirmed by transfecting other artery smooth muscle cells mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Artery Smooth Muscle Cell Avalanche Transfection Reagent. Human Artery Smooth Muscle Cell Avalanche Transfection Reagent can also be used on the following cells with high transfection efficiencies. Human Prostate Smooth Muscle Cell (PrSMC) Human Umbilical Vein Smooth Muscle Cell (HUVSMC) 786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days). The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.