Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Glycosylated disulfide-linked homodimer

Xeno-free BMP-4^HuXp is expressed in human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 34 kDa. This cytokine is produced in a serum-free, chemically defined media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 20 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 34 kDa, homodimer, glycosylated
• Formulation : 2x PBS + 6% Ethanol

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).