Product Description:

• Endotoxin-free
• Animal-derived product free
• High Activity
• Authentic Glycosylation

Xeno-free FGF-4^HuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 17 and 27 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Authentic glycosylation contributes to stability in cell growth media and other applications. FGF-4 plays an important physiologically role in the self-renewal of human embryonic stem cells. It also promotes stem cell proliferation. Furthermore, FGF-4 may also aid differentiation which additionally relies on context and concentration. The cytokine is greater than 95% pure.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤1.25 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 17 and 27 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS.