Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylation
• Available in Bulk

Xeno-free TPO^HuXp is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 80 to 85 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. C- terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 5 ng/mL EC50
• Purity: >95%
• Endotoxin: < 1 EU/µg
• Molecular Mass: 80 to 85 kDa, monomer, glycosylated.
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).