Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Authentic Glycosylation

Xeno-free Noggin^HuXp is expressed in an engineered human 293 cell expression system with serum- free, chemically defined media. The protein is a highly stable, authentically glycosylated, disulfide linked dimer. Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer (120-10C) consisting of two 206 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS-PAGE analysis under reducing conditions.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 25 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 65 kDa, homodimer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose dependent inhibition of rh-BMP-4 induced alkaline phosphate production by ATDC5 cells.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).