Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)
• Expressed as disulfide-linked heterodimer

Xeno-free IL-23^HuXp is expressed in human 293 cells as a heterodimeric glycoprotein composed of two disulfide-linked subunits (p40 cystine linked to p19). IL-23 has been shown to enhance proliferation of memory T cells. It also stimulates the production of IFN-gamma in NK cells, induces IL-17 production, and drives Th17 mediated responses. Furthermore, it is known that IL-23 takes a vital part in the inflammation process and that it is associated with auto immune diseases.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤ 30 ng/mL EC₅₀
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 55 kDa, heterodimer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose- dependent secretion of IL-17 in response to IL-23 using murine splenocytes.

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).