Product Description:
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1β, TNF-α, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive.
Technical Specifications:
- Species: Human
- Expression: E. coli Cell Expressed
- Activity: Typically ≤1.0 ng/mL ED50
- Purity: ≥98%
- Endotoxin: <1.0 EU/μg
- Molecular Mass: 12.6 kDa
- Formulation: 20mM Sodium Phosphate, pH 7.0
- Country of Origin: USA
Purity Confirmation:
This was determined by SDS-PAGE gel and HPLC analysis.
Activity Assay:
Determined by its ability to stimulate the proliferation of human TF-1 cells.
AA Sequence:
| MSPGPVPPST | ALRELIEELV | NITQNQKAPL |
| CNGSMVWSIN | LTAGMYCAAL | ESLINVSGCS |
| AIEKTQRMLS | GFCPHKVSAG | QFSSLHVRDT |
| KIEVAQFVKD | LLLHLKKLFR | EGRFN |
Reconstitution Buffer:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Slow to dissolve. Do not vortex.
Storage:
This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.
