Product Description:

• Endotoxin-free
• Animal-derived product free
• Lyophilized and Carrier Free (CF
• High Activity
• Authentic Glycosylation

Xeno-free LEFTY-1^HuXp is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 38 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤40 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Molecular Mass: 38 kDa, monomer, glycosylated
• Formulation: 10 mM Tris-HCl pH 7.4 + 50 mM NaCl + 0.5% CHAPS

Purity Confirmation:

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse chondrogenic cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).