首页 > Neuromics > Neuromics/FAS Ligand (CD95L)/GT15226/50 ug

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Neuromics/FAS Ligand (CD95L)/GT15226/50 ug

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GT15226-50ug

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Neuromics

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产品说明

Type: Goat IgG Applications: IHC; WB; E; NBE=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like AssaysSpecies Reactivity: HB=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; AllFormat: Affinity Purified - liquid Immunogen: Purified, CHO cell-derived, recombinant human Fas Ligand (rhFas Ligand). FAS Ligand, also known as CD95L or APT1LG1 , is a member of the TNF receptor family. The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing. Fas ligand-receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Figure 1-The biological activity of rhFas Ligand was measured by its ability to induce apoptosis of Jurkat cells (Cifone M.G. et al., 1994, J. Exp. Med. 180:1547). The ED50 of this effect is typically 0.2 - 0.5 ng/mL. Figure 2-To measure the ability of the antibody to neutralize the bioactivity of rhFas Ligand, rhFas Ligand was incubated with various concentrations of the antibody for 1 hour at 37° C. Following this preincubation period, Jurkat cells were added. The assay mixture in a total volume of 100 μL/well, containing antibody at the concentrations indicated, rhFas Ligand at 2 ng/mL and Jurkat cells at 5 x 104/mL, was incubated for 2 days at 37° C in a 5% CO2 humidified incubator. 3H-thymidine was added during the final 4 hours of the incubation. The cells were subsequently harvested onto a glass fiber filter and the 3H-thymidine incorporation into DNA was determined. The ND50 of this antibody is typically 2 - 8 ng/mL.

Neuromics

Neuromics公司是一家著名的神经科学产品和神经干细胞产品专业供应室，提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂等。

Our unique value is based on providing reagents that are thoroughly tested and characterized. We stand behind our products. If our reagents or cells do not meet expectations, we will fix it. We will either send equal replacement quantities or refund your money. Your satisfaction and feedback are important to our mutual success.

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