Type: Kit

Applications: E

E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays

Species Reactivity: H

B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All

Sensitive, Specific and Cost Effective

Interleukin-8 (IL-8) is a member of a family of structurally-related low molecular weight proinflammatory factors known as chemokines. IL-8 is produced by stimulated monocytes but not by tissue macrophages and Tlymphocytes. IL-8 is a non-glycosylated protein of 8 kDa (72 amino acids). It is produced by processing of a precursor protein of 99 amino acids

IL-8 induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. IL-8 is also known to be a potent promoter of angiogenesis

Figure: Figure 1. Serum level of IL-8. The results are mean and SEM (n = 34). #Statistically significant (Student t-test, p < 0.001). doi:10.4236/ojcd.2013.31002 Published Online March 2013

Suggested Application: Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.

Kit Components:·   Pre-Coated 96-Well Strip Microplate·   Wash Buffer·   Stop Solution·   Assay Diluent(s)·   Lyophilized Standard·   Biotinylated Detection Antibody·   Streptavidin-Conjugated HRP·   TMB One-Step SubstrateOther Materials Required:·     Distilled or deionized water·     Precision pipettes to deliver 2 μl to 1 ml volumes·     Adjustable 1-25 ml pipettes for reagent preparation·     100 ml and 1 liter graduated cylinders·     Tubes to prepare standard and sample dilutions·     Absorbent paper·     Microplate reader capable of measuring absorbance at 450nm·     Log-log graph paper or computer and software for ELISA data analysisProtocol Outline:1. Prepare all reagents, samples and standards as instructed in the manual.2. Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C.3. Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT.4. Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT.5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.

 

Figure: Box plot of the serum IL-6 level in the RA patients and control according to the IL-6 (−174 G/C) promoter polymorphism type. http://dx.doi.org/10.1016/j.ejr.2012.11.002