Agrisera/PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5/AS09 508/-蚂蚁淘生物


product information
Background		PIP2;5 protein is an aquaporins which facilitates the transport of water and small neutral molecules across cell membrane.
Immunogen		KLH-conjugated peptide derived from Oryza sativa PIP2-5 Q8GRI8
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format
Quantity		100 µl
Reconstitution
Storage		Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications		ELISA (ELISA), Western Blot (WB)
Related products		collection of antibodies to plasma membrane proteins AS09 504 \| anti-PIP1;3 \| aquaporin, plasma membrane intrinistic protein 1-3 AS09 505 \| anti-PIP1s \| aquaporin AS09 506 \| anti-PIP2;1 \| aquaporin, plasma membrane intrinistic protein 2-1 AS09 507 \| anti-PIP2;1 \| aquaporin, plasma membrane intrinistic protein 2-1 Plant protein extraction buffer Secondary antibodies
Additional information		0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient. Protocol for isolation of plasma membrane can be found here

application information
Recommended dilution		1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
Expected \| apparent MW		29.7 \| 26-29 kDa
Confirmed reactivity		Oryza sativa
Predicted reactivity		monocots including: Sorghum bicolor, Zea mays
Not reactive in		Arabidopsis thaliana, Hordeum vulgare
Additional information		Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC. Manufactured by Operon Biotechnologies.
Selected references		Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

application example

5 µg of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;5 antibodies (AS09 508, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

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