Catalog Number

Size

13822P-5

5ug

13822P-20

20ug

13822P-1000

1000ug

Catalog Number

Size

13822P-5

5ug

13822P-20

20ug

13822P-1000

1000ug

ORDERING INFORMATION

Formulation: Sterile-filtered clear solution (0.25mg/ml) in 20mM Tris-HCl, pH 8.0, 150mM NaCl,1mM DTT, 40%

glycerol. Purified by proprietary chromatographic techniques.

Annexin 7 is a member of the annexin family of calcium-dependent phospholipids binding proteins. It includes a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter is composed of alternating hydrophobic and hydrophilic segments. Analysis of this protein suggests that Annexin 7 is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity, and membrane fusion.

Recombinant Human Annexin A7 produced in E. coli is a single, non- glycosylated polypeptide containing amino acids 1-466 with a molecular weight of 52.9kDa. It includes a 24 amino acid His-tag at the N-terminus.

Source: Escherichia coli Purity: Greater than 85% as determined by SDS-PAGE. Accession number: P20073.3 Amino acid sequence: MGSSHHHHHH SSGLVPRGSH

MGSHMSYPGY PPTGYPPFPG YPPAGQESSF PPSGQYPYPS GFPPMGGGAY PQVPSSGYPG AGGYPAPGGY PAPGGYPGAP QPGGAPSYPG VPPGQGFGVP PGGAGFSGYP QPPSQSYGGG PAQVPLPGGF PGGQMPSQYP GGQPTYPSQP ATVTQVTQGT IRPAANFDAI RDAEILRKAM KGFGTDEQAI VDVVANRSND QRQKIKAAFK TSYGKDLIKD LKSELSGNME ELILALFMPP TYYDAWSLRK AMQGAGTQER VLIEILCTRT NQEIREIVRC YQSEFGRDLE KDIRSDTSGH FERLLVSMCQ GNRDENQSIN HQMAQEDAQR LYQAGEGRLG TDESCFNMIL ATRSFPQLRA TMEAYSRMAN RDLLSSVSRE FSGYVESGLK TILQCALNRP AFFAERLYYA MKGAGTDDST LVRIVVTRSE IDLVQIKQMF AQMYQKTLGT MIAGDTSGDY RRLLLAIVGQ

Store at 4oC if entire vial will be used within 2-4 weeks. Store at -20oC for longer periods of time. For long-term storage, addition of 0.1% human serum albumin or bovine serum albumin is recommended. Avoid multiple freeze- thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.