ORDERING INFORMATION

Catalog No.: 43051

Format: 100ul at 1.0mg/ml in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Affinity-purified on phosphopeptide; non-phosphopeptide- reactive antibodies were removed by chromatography on non-phosphorylated peptide.

BACKGROUND

Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.

SPECIFICATION SUMMARY

Antigen: Peptide sequence that includes phosphorylation site of threonine 645 (P-A-T(p)-I-K) derived from human STAT6 and conjugated to KLH.

Accession no.: P42226, NP_003144.3

Host Species: Rabbit

Specificity: This antibody detects endogenous human STAT6 only when phosphorylated at threonine 645.

APPLICATION

Immunoblotting: use at dilution of 1:500-1:1,000. A band of~110kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

Detection of STAT6 (phospho-Thr645) in extracts of HeLa cells treated with IL-4 or calf intestinal phosphatase.

These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.

Detection of STAT6 (phospho-Thr645) in paraffin- embedded human breast carcinoma tissue.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

This antibody is stable for at least one (1) year at -20oC. Can be stored at 4oC for short-term use.

For in vitro investigational use only. Not intended for therapeutic or diagnostic applications.