EndoPDI Monoclonal AntibodyORDERING INFORMATIONCatalog No.: 56458 (clone 2E7/7) Size: 100ug in PBS (1mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
BACKGROUNDEndothelial protein disulfide isomerase (EndoPDI) is a thiredoxin member of the protein disulfide isomerase family of chaperones. This enzyme is localized in the endoplasmic reticulum in primary endothelial cells but colocalizes with nucleoli in the nuclei of breast, colon, and kidney cancer cells. EndoPDI mRNA and protein expression is iduced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia.
SPECIFICATION SUMMARYAntigen: Synthetic peptide corresponding to aa 52-63 (ADGEDGQDPHSK) of human EndoPDI (accession no. NP_001139021).Host Species: MouseAntibody Class: IgG2aSPECIFICITYThis antibody recognizes human EndoPDI.
APPLICATIONSImmunoblotting: use at 1ug/ml. A band of
~48kDa is detected.
Positive control: heat-shocked HeLa cell lysate.
These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
DILUTION INSTRUCTIONSDilute in PBS or medium which is identical to that used in the assay system.
STORAGE AND STABILITYThis antibody is stable for at least one (1) year at -20oC. Avoid repeated freeze- thaw cycles.
For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.
