Cat-Nr.100-071Size20 µgPrice170 €SourceE. coliFormulationlyophilizedPurity Confirmation> 98% by SDS-PAGE & HPLC analysesLength [aa]123Molecular Weight13.4 kDaEndotoxin Levels< 0.1 ng/µg of protein (<1EU/µg)Biological ActivityDetermined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10.0 ng/ml.Species ReactivityMouse, Pig, Shark, Human, Hagfish, SquidBuffer0.4x PBS, pH 7.4ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at ‑20°C to -80°C.Stability and StorageThe lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. SynonymsMDK; MK; NEGF2 DescriptionMidkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages, and by signaling through the ALK receptor it exerts angiogenic, growth and survival activities during tumorgenesis. Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.Protein SequenceVAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKDUniprot IDP21741Protein RefSeqNP_001012333mRNA RefSeqNM_001012333