TestedandCertifiedforinvivoimaging(See\"LuciferinFAQ\"inadditionalinformation)Luciferinisacommonbioluminescentreporterusedforinvivoimagingoftheexpressionofluciferase.ThiswatersolublesubstratefortheFireflyluciferaseenzymeutilizesATPandMg2+asco-factorstoemitacharacteristicyellow-greenemissioninthepresenceofoxygen,whichshiftstoredlightinvivoat37 C.ThroughtheutilizationofATP,thereactioncanbefurtherusedtoindicatethepresenceofenergyorlifeinordertofunctionasalife-deathstain.LuciferinisacommonreagentusedthroughouttheBiotechnologyfieldandspecificallyforinvivoimaging.Luciferaselabeledtumorcells,stemcells,orinfectiousdiseasesareofteninoculatedintoresearchanimalssuchasratsormiceforinvestigation.TheinjectionofLuciferinallowsforthereal-time,non-invasivemonitoringofdiseaseprogressionand/ordrugefficacyinthesemodelsystemsthroughBioluminescenceImaging(BLI).Luciferinisalsocommonlyusedforinvitroresearch;includingluciferaseandATPassays,genereporterassays,highthroughputsequencingandvariouscontaminationassays.FireflyLuciferinisidenticaltoBeetleLuciferin.(See\"AdditionalInformation\"forstructures.)ProductSpecifications:D-Luciferin,Potassium S alt4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacidpotassium sal tMOLECULARBIOLOGYGRADE(PremiumPure)Formula:KC11H7N2O3S2MW:318.42g/mol
Color:LightyellowpowderPurity: 99%pure.(QualityverifiedbynineindependentcriteriaincludingHPLCandFTIR.)Storage/Handling:Storedessicatedat-20 C.Protectfromlight.PubChemChemicalID:44134804
