More Information Product Details
Synonyms TRPM-2; Apolipoprotein J; APO-J; CLI; CLU; SGP-2 |
Product Type Protein |
Properties
Source/Host E. coli |
Sequence Human nuclear clusterin (aa 23-449) is fused at the C-terminus to a His-tag. |
Crossreactivity Human |
MW ~45kDa (SDS-PAGE) |
Purity ≥90% (SDS-PAGE) |
Endotoxin Content |
Concentration 1mg/ml |
Formulation Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. |
Other Product Data UniProt link P10909: Clusterin (human) [Precursor] |
Shipping and Handling
Shipping BLUE ICE |
Short Term Storage +4°C |
Long Term Storage -20°C |
Handling Advice After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles. |
Use/Stability Working aliquots are stable for up to 3 months when stored at -20°C. |
Documents
MSDS Download PDF |
Product Specification Sheet
Datasheet Download PDF |
Clusterin shares homology with the small heat shock protein family of molecular chaperones. The mature secreted form of the protein is a glycosylated, 80-kDa disulfide-linked heterodimer of alpha and beta subunits (produced by internal cleavage). Clusterin is expressed in virtually all tissues and found in all human fluids. It is involved in numerous physiological processes important for carcinogenesis and tumor growth, including apoptotic cell death, cell cycle regulation, DNA repair, cell adhesion, tissue remodeling, lipid transportation, membrane recycling, and immune system regulation. Clusterin also exists as a nuclear protein. The secreted form of Clusterin has extracellular chaperone and anti-apoptotic activities while the nuclear form acts as a proapoptotic factor.
Product References
Clusterin induces matrix metalloproteinase-9 expression via ERK1/2 and PI3K/Akt/NF-κB pathways in monocytes/macrophages: Y-J. Shim, et al.; J. Leuk. Biol. 90, 761 (2011)Clusterin Induces MUC5AC Expression via Activation of NF-κB in Human Airway Epithelial Cells: C.H. Bae, et al.; Clin. Exp. Otorhinolaryngol. ahead of print (2018)