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Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE

Kit

Quantitative ELISA

Detects mouse caspase-1 (proform as well as p10 and p20 domain). Does not detect human caspase-1.

Mouse

1 x 96 wells

33pg/ml

0.15ng/ml to 10ng/ml

Cell Culture SupernatantPlasmaSerum

Sandwich

Colorimetric

BLUE ICE

+4°C

+4°C

After standard reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Plate and reagents should reach room temperature before use.

12 months after the day of manufacturing. See expiry date on ELISA Kit box.

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Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals.This Caspase-1 (mouse) ELISA Kit can be used to measure caspase-1 (mouse) in cell culture supernatants, serum and plasma. It is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.Product References

Pharmacologic inhibition of IκB kinase activates immediate hypersensitivity reactions in mice: D. Miyazaki, et al.; Am. J. Pathol. 183, 96 (2013)