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Active Motif(艾跃)/3-Methylcytosine (3-mC) antibody (pAb)/61112/10µg
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ApplicationsApplicationNotesValidatedApplications:DB:0.1-1µg/mldilutionPublishedApplications:ICC/IFDotBlotPleaseseereferencesformoreinformation.Individualoptimizationmayberequired.ImmunogenThis3-methylcytosineantibodywasraisedagainst3-methylcytidineconjugatedtoKLHandrecognizes3-methylcytosine.BufferPurifiedrabbitIgGinPBSwith30%glyceroland0.035%sodiumazide.Sodiumazideishighlytoxic.References3-Methylcytosine(3-mC,3-methylcytidine)antibodytestedbydotblotanalysis.BSAconjugatednucleosides(startingat10ngasindicated)werespottedontoPVDFmembraneandblottedwith3-methylcytidineantibodyatadilutionof1:10,000.Lane1:3-methylcytidine.Lane2:Cytidine.Lane3:5-methylcytidine.Lane4:5-hydroxymethylcytidine.BackgroundMethylationofDNAcanoccurnon-enzymaticallyatthenitrogen-threeofthecytosinebasethroughspontaneousexposuretoendogenousS-adenosylmethionine(SAM).Theresulting3-methylcytosine(3-mC)ismutagenicandmustberepaired,whichoccursinhumansthroughthebaseexcisionrepair(BER)ordealkylationviahumanhomologuesoftheE.coliAlkBprotein.3-methylcytosineispresentinhumancelllinesandincreasedlevelsof3-mCimpairproliferation.CompatIBLeSecondaryAntibodiesActiveMotifhasdevelopedavarietyofhigh-qualitysecondaryantibodiesformostapplications,withanti-rabbitandanti-mouseconjugatestoabroadlineofhigh-qualityfluorescentmolecules,aswellashorserADIshperoxidase(HRP).VisitourSecondaryAntibodyConjugatespagetofindtheperfectsecondariesforimmunofluorescenceexperimentswith3-Methylcytosine(3-mC)antibody(pAb).StorageSomeproductsmaybeshippedatroomtemperature.ThiswillnotaffecttheirstABIlityorperformance.Uponreceipt,unconjugatedantibodiesmaybestoredat-20°Cforupto2years.Fluorophore-&enzyme-conjugatedantibodiesshouldbestoredat4°C.Fluorophore-conjugatedantibodiesshouldbeprotectedfromlight.Keepreagentsonicewhennotinstorage;toavoidrepeatedfreeze/thawcycles,werecommendaliquotingitemsthatwillbestoredfrozenintosingle-usefractionspriortofreezing.GuaranteeThisproductisguaranteedfor6monthsfromdateofreceipt.Thisproductisforresearchuseonlyandisnotforuseindiagnosticprocedures.ApplicationKeyChIP=ChromatinImmunoprecipitation;DB=DotBlot;ELISA=Enzyme-linkedImmunosorbentAssay;EMSA=ElectrophoreticMobilityShiftAssayFC=FlowCytometry;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;MeDIP=Methyl-DNAImmunoprecipitation;TR-FRET=Time-ResolvedFluorescenceResonanceEnergyTransfer;WB=WesternBlotting
Active Motif 公司是一家致力于研发和生产核细胞分子生物学和表观遗传学研究用试剂的专业公司,也是世界上最大的转录因子试剂生产商和 ChIP-seq 外包服务供应商。Active Motif 的产品涉及:表观遗传学相关抗体、Chip 级别抗体、重组识别子和催化酶、预甲基化修饰的重组组蛋白、多聚核小体和生物素标记核小体;以及各类染色质分析、转录因子表达调控分析和 DNA 甲基化分析的试剂盒等。不仅如此,Active Motif 还为研究者提供专业的表观遗传学服务,特别是 Chip-seq 的外包服务。公司总部位于美国加州,目前在欧洲,日本,中国设有分公司。产品销售范围覆盖全球 30 多个国家和地区。
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