类型：重组蛋白
NewRD2725901000.1 mgSoluble高级糖基化终产物受体人类大肠杆菌类型：重组蛋白
RD1721161000.1 mg产品和服务COVID-19免疫分析下一代测序microRNA和sncRNA蛋白质抗体免疫组化内毒素检测和去除产品细胞外分析Matrix AssaysDNA/RNA Extraction Instruments/KitsqPCR Kits/Enzymes/ReagentsLAMP TestsBioVendor 分析测试服务你在这里：首页产品和服务蛋白质辛卡利特-蛋白质NewRP17827400055 mgNewRP178274002525 mg产品和服务COVID-19免疫测定下一代测序microRNA和sncRNA蛋白质抗体免疫组化内毒素检测和去除产品基于细胞的检测细胞外基质检测DNA/ RNA Extraction Instruments/KitsqPCR Kits/Enzymes/ReagentsLAMP TestsBioVendor 分析测试服务你在这里：首页产品和服务 ProteinssFRP4 -Proteins产品和服务COVID-19ImmunoassaysNext Generation SequencingmicroRNA & sncRNAsProteinsAntibodiesImmunohistochemistryEndoxin Detection & Removal ProductsCell-Based AssaysExtracellular Matrix AssaysDNA/RNA Extraction Instruments/KitsqPCR Kits/Enzymes/ReagentsLAMP TestsBioV endor 分析测试服务你在这里：首页产品和服务蛋白质 Serglycin -蛋白质产品和服务COVID-19免疫测定下一代测序microRNA和sncRNA蛋白质抗体免疫组织化学内毒素检测和去除产品基于细胞的测定细胞外基质测定DNA/RNA提取仪器/试剂盒qPCR试剂盒/酶/试剂LAMP测试生物供应商分析测试服务你在这里：首页产品和服务蛋白质分泌珠蛋白家族 3A 成员 2 -蛋白质产品和服务COVID-19免疫测定下一代测序microRNA 和 sncRNAs蛋白质抗体免疫组织化学内毒素检测和去除产品基于细胞的测定细胞外基质测定DNA/RNA 提取仪器/KitsqPCR 试剂盒/酶/Rea gentsLAMP 测试BioVendor 分析测试服务你在这里：首页产品和服务蛋白质分泌珠蛋白家族 1D 成员 1-蛋白质产品和服务COVID-19免疫测定下一代测序microRNA 和sncRNAs蛋白质抗体免疫组织化学内毒素检测和去除产品基于细胞的测定细胞外基质测定DNA/RNA 提取仪器/KitsqPCR 试剂盒/酶/Rea gentsLAMP 测试BioVendor 分析测试服务你在这里：首页产品和服务蛋白质硬化蛋白 -蛋白质硬化蛋白人 HEK293类型：重组蛋白
RD172244100-HEK0.1 mg产品和服务COVID-19免疫测定下一代测序microRNA和sncRNA蛋白质抗体免疫组化内毒素检测和去除ProductsCell-Based AssaysExtracellular Matrix AssaysDNA/RNA Extraction Instruments/KitsqPCR Kits/Enzymes/ReagentsLAMP TestsBioVendor 分析测试服务- Regulatory status:RUO
- Type:Recombinant
- Source:E. coli
- Other names:TP53-induced glycolysis and apoptosis regulator (TIGAR)
- Species:Human
Type
Recombinant
Description
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2–6 bisphosphate (F-2–6-BP) to fructose-6-phosphate and inorganic phosphate. F-2–6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2–6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary, as well as transformed, cells. Recombinant Human TIGAR-TAT expressed in E. coli is a 31.6 kDa protein containing 283 amino acid residues, including the 269 residues of full-length TIGAR fused to a 14-residue C-terminal peptide containing the TAT transduction domain (GGGYGRKKRRQRRR).
Amino Acid Sequence
ARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETRGGGYGRKKRRQRRR
Source
E. coli
Purity
95%
Biological Activity
Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1–5.0 µg/ml, protects U2OS cells from apoptosis induced by hydrogen peroxide.
Endotoxin
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.
Storage/Expiration
–20°C
