Fitzgerald/UNG antibody/10R-6237/100 ul
OVERVIEW:
Synonyms | Monoclonal UNG antibody, Anti-UNG antibody, Uracil-DNA glycosylase antibody, UDG antibody, UNG1 antibody, HIGM4 antibody, UNG15 antibody, Monoclonal UNG antibody, Anti-UNG antibody, Uracil-DNA glycosylase antibody, UDG antibody, UNG1 antibody, HIGM4 antibody, UNG15 antibody |
Cross Reactivity | Human, Monkey |
Applications | IHC, WB |
Immunogen | UNG antibody was raised in mouse using a full length recombinant protein of human UNG (NP_550433) produced in HEK293T cells, as the immunogen. |
SPECIFICATIONS:
Host | Mouse |
Clone | 1G2 |
Isotype | IgG2b |
Method of Purification | UNG antibody was purified by affinity chromatography. |
Form & Buffer | Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Concentration | 500 ug-1 mg/ml |
STORAGE & SAFETY:
Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Usage Assay Information:
Usage Recommendations | IHC: 1:150, WB: 1:500-2000 |
GENERAL INFORMATION:
Biological Significance | UNG is one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is toprevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating thebase-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. |
