Fitzgerald/DFFA antibody/10R-1649/50 ug
OVERVIEW:
Synonyms | Monoclonal DFFA antibody, Anti-DFFA antibody, ICAD antibody, DFF1 antibody, DFF-45 antibody, DNA fragmentation factor alpha polypeptide antibody |
Specificity | Human DFFA |
Cross Reactivity | Human. Other species not studied |
Applications | Dot Blot |
Immunogen | DFFA antibody was raised in mouse using recombinant Human Dna Fragmentation Factor, 45Kda, Alpha Polypeptide (Dffa) |
SPECIFICATIONS:
Host | Mouse |
Clone | 2262C2a |
Isotype | IgG1 |
Method of Purification | DFFA antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
Form & Buffer | DFFA antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ). |
Concentration | 100 ug/ml |
STORAGE & SAFETY:
Storage | Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing. |
Biohazard Information | This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv |
Usage Assay Information:
Usage Recommendations | To be optimized by the end user. |
GENERAL INFORMATION:
Biological Significance | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40 kDa (DFFB) and 45 kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
