首页 > NSJ Bio > NSJ Bio/Anti-CD45RO Antibody [clone SPM125] (V2420)/7 ml/V2420IHC-7ML

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NSJ Bio/Anti-CD45RO Antibody [clone SPM125] (V2420)/7 ml/V2420IHC-7ML

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V2420IHC-7ML

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NSJ Bio

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DescriptionRecognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.Application NotesDue to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.ImmunogenCultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.StorageThe anti-CD45RO antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder. References (1) Related Products1. View all CD45RO antibodies2. View all CD45 antibodies

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