Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human and Mouse. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name BCL6/850 Purity Protein G affinity chromatography UniProt P41182 Localization Nuclear Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 1-2ug/mlImmunohistochemistry (FFPE) : 0.1-0.3ug/ml for 30 min at RT (1)Prediluted IHC only format : incubate for 30 min at RT (2) Limitations This Bcl6 antibody is available for research use only. Description:Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.Immunogen:Recombinant human protein was used as the immunogen for the Bcl6 antibody.Storage:Store the Bcl6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).