Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human, Mouse. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name ARBC6-1 Purity Protein G affinity chromatography UniProt P41182 Localization Nuclear Applications Immunofluorescence : 1-2ug/mlImmunohistochemistry (FFPE) : 0.1-0.3ug/ml for 30 min at RT (1)Prediluted IHC only format : incubate for 30 min at RT (2) Limitations This Bcl6 antibody is available for research use only. Description:Bcl6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. [UniProt]Immunogen:Recombinant human protein was used as the immunogen for the Bcl6 antibody.Storage:Store the Bcl6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).