Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1 Clone Name C4D203 Purity Protein G affinity chromatography UniProt P0C0L4 , P0C0L5 Localization Intracytoplasmic vacuoles of endothelial cells and secreted Applications ELISA : 1-2ug/ml order BSA-free format)Immunofluorescence : 0.5-1ug/ml Limitations This C4d antibody is available for research use only. Description:This mAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.Application Notes:Optimal dilution of the C4d antibody should be determined by the researcher.Immunogen:Recombinant human Complement 4d protein was used as the immunogen for the C4d antibody.Storage:Store the C4d antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).