Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG2b, kappa Clone Name C11b/660 Purity Protein G affinity chromatography Buffer 1X PBS, pH 7.4 Gene ID 3684 Localization Cell surface, cytoplasm Applications Functional Testing : order azide-free formatFlow Cytometry : 0.5-1ug/million cellsImmunofluorescence : 0.5-1ug/ml Limitations This CD11b antibody is available for research use only. Description:CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin alpha-M (CD11b) combines with the Integrin beta-2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin alpha-M/beta-2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.Application Notes:The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.Immunogen:Recombinant human CD11b protein was used as the immunogen for this antibody.Storage:Store the CD11b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).