Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name BLCAM/1795 Purity Protein G affinity chromatography UniProt P20273 Localization Cell surface, cytoplasmic Applications ELISA : 2-4ug/ml; order BSA free formulation for coatingFlow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 1-2ug/mlWestern blot : 0.5-1ug/mlImmunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Limitations This CD22 antibody is available for research use only. Description:Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.Application Notes:Optimal dilution of the CD22 antibody should be determined by the researcher.Immunogen:Amino acids 52-178 from the human protein were used as the immunogen for the CD22 antibody.Storage:Store the CD22 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).