Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide freeData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Species Reactivity Human. Other species not known. Format Purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Protein A affinity chromatography Buffer 1X PBS, pH 7.4 Gene ID 925 Localization Cell surface Applications Flow Cytometry : 1-2ug/million cellsImmunofluorescence : 1-2ug/ml Limitations This CD8a antibody is available for research use only. Description:CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha-beta) or as a homodimer (CD8 alpha-alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8 alpha antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.Application Notes:The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.Immunogen:Human CD8 recombinant fragment corresponding to aa 200-250 was used as the immunogen for this antibody.Storage:Store the CD8a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).