Formulation: Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*Data:Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide freeData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human, Mouse, Rat. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name CDH1/1122 Purity Protein G affinity chromatography UniProt P12830 Localization Cytoplasmic, membranous Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 0.5-1ug/mlWestern blot : 0.5-1ug/mlImmunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RTPrediluted IHC only format : incubate for 30 min at RT (1) Limitations This CDH1 antibody is available for research use only. Description:Recognizes a protein of 80-120 kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. CDH1 plays an important role in epithelial cell adhesion. Decreased expression is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin/CDH1 +) and lobular (E-cadherin/CDH1 -) breast carcinomas. It may also help in diagnosis of mesothelioma.Immunogen:A recombinant fragment (119 amino acid residues around aa 400-500) from the humn protein was used as the immunogen for the CDH1 antibody.Storage:Store the CDH1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).