Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide freeData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG2b, kappa Clone Name CELA3B/1811 Purity Protein G affinity chromatography UniProt P08861 Localization Cytoplasmic Applications Western blot : 0.5-1ug/ml Limitations This CELA3B antibody is available for research use only. Description:This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.Application Notes:Optimal dilution of the CELA3B antibody should be determined by the researcher.Immunogen:A partial recombinant protein (aa 82-238) was used as the immunogen for the CELA3B antibody.Storage:Store the CELA3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).