Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI waterData: Availability 1-3 business days Species Reactivity Human, Rat Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide Gene ID 9075 Applications Western blot : 0.5-1ug/mlIHC (FFPE) : 0.5-1ug/ml Limitations This Claudin 2 antibody is available for research use only. Description:Claudin 2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.Application Notes:The stated application concentrations are suggested starting points. Titration of the Claudin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:Human partial recombinant protein (AA 38-230) was used as the immunogen for this Claudin 2 antibody.Storage:After reconstitution, the Claudin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.