Formulation: 0.5mg/ml if reconstituted with 0.2ml sterile DI waterData: Availability 1-3 business days Species Reactivity Human, Mouse, Rat Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Antigen affinity Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal UniProt P26196 Applications Western blot : 0.5-1ug/ml Limitations This DDX6 antibody is available for research use only. Description:DEAD/H box 6, also known as RCK, HLR2 or p54, is an enzyme that in humans is encoded by the DDX6 gene. It belongs to the DEAD box family of putative RNA helicases that contain a characteristic asp-glu-ala-asp(DEAD) box motif(Seto et al., 1995). Tunnacliffe et al.(1993) assigned the gene more precisely using a panel of sequence tagged sites(STSs) representing 30 markers previously assigned to 11q23. Using mass spectroscopy, Fenger-Gron et al.(2005) found that DDX6, EDC3 (YJDC), and HEDLS (RCD8) coimmunopurified with DCP1A and DCP2 from HEK293 cell lysates. Overexpression of DCP2, DDX6, or EDC3 in HeLa cells reduced the association of endogenous DCP1A and XRN1 with cytoplasmic P bodies.Application Notes:The stated application concentrations are suggested starting amounts. Titration of the DDX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:An amino acid sequence from the C-terminus of human DEAD/H box 6 (YDDRFNLKSIEEQL) was used as the immunogen for this DDX6 antibody (100% homologous in human, mouse and rat).Storage:After reconstitution, the DDX6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.