Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide freeData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human and Mouse. Not suitable for Rat sample testing. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name CDH1/1121 Purity Protein G affinity chromatography UniProt P12830 Localization Cytoplasmic, membranous Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 0.5-1ug/mlWestern blot : 0.5-1ug/mlImmunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RTPrediluted IHC only format : incubate for 30 min at RT (1) Limitations This E-Cadherin antibody is available for research use only. Description:Recognizes a protein of 80-120kDa, identified as E-cadherin/CDH1. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.Immunogen:A recombinant fragment (119 amino acid residues around aa 400-500) from the human protein was used as the immunogen for the E-Cadherin antibody.Storage:Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).