Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azideData: Availability 1-3 business days Species Reactivity Human Predicted Reactivity Mouse Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity UniProt P00533 Localization Cytoplasmic, membranous Applications Western blot : 1:1000 Limitations This Epidermal growth factor receptor antibody is available for research use only. Description:EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Application Notes:Titration of the Epidermal growth factor receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:A portion of amino acids 956-985 from the human protein was used as the immunogen for this Epidermal growth factor receptor antibody.Storage:Aliquot the Epidermal growth factor receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.