Formulation: 1 mg/ml in 1X PBS; BSA free, sodium azide freeData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Species Reactivity Human. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG1, kappa Clone Name LEC67 Purity Protein G affinity chromatography Buffer 1X PBS, pH 7.4 Gene ID 6360 Localization Secreted (extracellular) Applications ELISA : order BSA-free format Functional testing : order BSA/azide free format Limitations This HCC-4 antibody is available for research use only. Description:HCC-4, also called LEC, shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelo-suppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant HCC-4 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. HCC-4 (LEC) is predominantly expressed in liver, but also found in spleen and thymus. It is highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones. The ability of HCC-4 (LEC) to markedly improve recognition of poorly immunogenic cells suggests that it could be an effective component of anti-tumor vaccines.Application Notes:The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.Immunogen:Recombinant human protein was used as the immunogen for this HCC-4 antibody.Storage:Store the HCC-4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).