Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azideData:Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azideData: Availability 1-3 business days Species Reactivity Mouse Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity UniProt P70424 Applications Western blot : 1:500-1000 Limitations This HER2/NEU antibody is available for research use only. Description:Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).Application Notes:Titration of the HER2/NEU antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:This mouse HER2/NEU antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1142-1176 amino acids from the human region of mouse Her2/Erbb2.Storage:Aliquot the HER2/NEU antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.