Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData:Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azideData: Availability 1-3 business days Species Reactivity Human and Mouse. Other species not known. Format Purified Clonality Monoclonal (mouse origin) Isotype Mouse IgG2b, kappa Clone Name KP10 + KIP2/880 Purity Protein G affinity chromatography UniProt P49918 Localization Nuclear Applications Flow Cytometry : 0.5-1ug/million cells in 0.1mlImmunofluorescence : 0.5-1ug/mlImmunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT (1)Prediluted IHC only format : incubate for 30 min at RT (2) Limitations This p57 antibody cocktail is available for research use only. Description:Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.Immunogen:Recombinant human protein was used as the immunogen for the p57 antibody cocktail.Storage:Store the p57 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).