Formulation: In 1X PBS, pH 7.4, with 0.09% sodium azideData: Availability 1-3 business days Species Reactivity Human Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Antigen affinity UniProt Q01094 Applications Western blot : 1:1000IHC (Paraffin) : 1:50-1:100 Limitations This phospho-E2F1 antibody is available for research use only. Description:E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.Application Notes:Titration of the phospho-E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.Immunogen:This phospho-E2F1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS337 of human E2F1.Storage:Aliquot the phospho-E2F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.